The process of obtaining intravenous (IV) access, Vein puncture, is an everyday invasive procedure in medical settings and there are more than one billion vein puncture related procedures like blood draws, peripheral catheter insertions, intravenous therapies, etc. performed per year. Excessive vein punctures are both time and resource consuming events causing anxiety pain and distress in patients or can lead to severe harmful injuries. The major problem faced by the doctors today is difficulty in accessing veins for intra-venous drug delivery & other medical situations. There is a need to develop vein detection devices which can clearly show veins. This project deals with the design development of non-invasive subcutaneous vein detection system and is implemented based on near infrared imaging and interfaced to a laptop to make it portable. A customized CCD camera is used for capturing the vein images and Computer Software modules (Open-CV) is used for the processing.